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Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only
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pDB016
Plasmid#99220PurposeAccessory plasmid for aaRS PACE; negative selectionDepositorInsertPT7 [SD8] gIII-neg
UseTagsExpressionBacterialMutationPromoterPT7Available sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-sfGFP
Plasmid#51562PurposeExpression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)DepositorInsertsuperfolder GFP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b Tau Cys
Plasmid#108867PurposeExpresses human Tau with a mutated cysteineDepositorInsertTau (MAPT Human)
UseTagsExpressionBacterialMutationI260C C291A C322A to allow maleimide dye labellingPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRE135
Plasmid#118489Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046828
Plasmid#145742PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a_HIV-RT
Plasmid#153311PurposeBacterial expression of HIV reverse transcriptaseDepositorInsertHIV Reverse Transcriptase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSetB-Peredox
Plasmid#73806PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tagDepositorInsertPeredox
UseTagshis-tag to facilitate purificationExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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XOR gate-V2.0
Plasmid#44453DepositorInsertXOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcher-sfGFP
Plasmid#107420PurposeFor production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
UseTagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationPromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig2
Plasmid#64842PurposeA lentiviral vector for the expression of mouse Olig2 under doxycyline controlDepositorInsertOlig2 (Olig2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLS.402
Plasmid#187202PurposeExpresses retron Eco1 RTDepositorInsertRetron Eco1 Reverse Transcriptase
UseSynthetic BiologyTagsExpressionBacterialMutationPromotermphRAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET14b – E. coli Exonuclease I
Plasmid#104552Purposebacterial expression of Exonuclease IDepositorInsertExonuclease I (sbcB E.coli)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only