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Plasmid#110203PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interestDepositorInsertenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
UseTagseGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Salmonella MTAN
Plasmid#64041PurposeExpression of S. enterica MTAN in E. coliDepositorInsertMethylthioadenosine nucleosidase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB5k-GFP
Plasmid#35354DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PHGDH-C234S
Plasmid#107724PurposeExpression of PHGDH C234S in bacteriaDepositorInsertPHGDH (PHGDH Human)
UseTagsExpressionBacterialMutationC234SPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC236
Plasmid#216368PurposexhNup155-Nb2i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb2i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC235
Plasmid#216372PurposexhNup155-Nb3i (+2Cys) Nanobody productionDepositorInsertxhNup155-Nb3i (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04163
Plasmid#216373PurposexhNup214-Nb1t (+2Cys) Nanobody productionDepositorInsertxhNup214-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-scSUMOExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC234
Plasmid#216357PurposexhNup93-Nb3t (+2Cys) Nanobody productionDepositorInsertxhNup93-Nb3t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC227
Plasmid#216351PurposexhNup35-Nb1t (+3Cys) Nanobody productionDepositorInsertxhNup35-Nb1t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
Plasmid#205161Purposeexpress β-ACTIN-3′UTR-24xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD48-daf-2 RNAi
Plasmid#34834DepositorInsertdaf-2 (daf-2 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 WT
Plasmid#174447PurposeExpress GFP-tagged RNase H1 WT in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
UseTagsHis-tag, GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa K44A
Plasmid#22197DepositorInserthuman Dynamin 1aa K44A (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationK44APromoterAvailable sinceNov. 2, 2009AvailabilityAcademic Institutions and Nonprofits only