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Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorArticleTypeEmpty backboneUseTagsPAmCherry1ExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
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pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
UseTags3x-FLAGExpressionBacterialMutationPromoterpTetAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-PfAgo_F
Plasmid#146265PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
UseTagsExpressionBacterialMutationone silent T1704C and one non silent mutation A90…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
SB_loop
Plasmid#110103PurposepBXPHM3 with scaffold Sybody of the loop libraryDepositorInsertscaffold Sybody of the loop library
UseTagsHisExpressionBacterialMutationPromoterPBADAvailable sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAmCherry1
Plasmid#31931DepositorInsertPAmCherry1
UseTagsHisExpressionBacterialMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET.GltI253-cpGFP.L1LV/L2NP
Plasmid#41733Purposea single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expressionDepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterT7Available sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMN252
Plasmid#32177DepositorInsertrpsL, FRT-hyg-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU1701
Plasmid#14461DepositorInsertgfp+
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSANG14-3F
Plasmid#39265DepositorTypeEmpty backboneUseTags6xHis, Alkaline phosphatase, pelB leader, and tri…ExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-EGFP-PRC1
Plasmid#167540PurposeFor expression of His6-TEV-EGFP-PRC1 in bacteria.DepositorInsertPRC1 (PRC1 Human)
UseTagsEGFP and His6 tagExpressionBacterialMutationPromoterT7Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA tag, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorInsertCullin 2 (CUL2 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only