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pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Use

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Use

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA13a-RFP

Plasmid

#126086

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23102

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB

Plasmid

#162572

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_10xHisTEVATG5_ATG12

Plasmid

#190861

Purpose

Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG12 (ATG12 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ReactionC_pBIG1a_ATG7_ATG10

Plasmid

#190860

Purpose

Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG7 (ATG7 Human)
ATG10 (ATG10 )

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lba_Cas13a_WT

Plasmid

#91867

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbaCas13a (LbaC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSALECT-EcoBam

Plasmid

#59705

Purpose

Folding reporter with ampicillin resistance as a readout

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-nanoHER2-SpyTag003

Plasmid

#216312

Purpose

Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

nanoHER2-SpyTag003

Use

Tags

His6 tag and SpyTag003

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKEN GFP mut2

Plasmid

#20409

Depositor

Stanley Falkow, Raphael Valdivia

Article

Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

S65A V68L S72A

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAA3.stelN

Plasmid

#112011

Purpose

Expresses TelN enzyme

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

TelN

Use

Tags

strep and his

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28sumo-MDA5-Full-length

Plasmid

#210826

Purpose

protein expression in ecoli

Depositor

Anna Pyle

Article

Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

Insert

MDA5 (IFIH1) Full Length Protein (IFIH1 Human)

Use

Tags

Expression

Bacterial

Mutation

NA

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:D70E

Plasmid

#79164

Purpose

Expresses constitutively active WspR D70E under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Use

Tags

Expression

Bacterial

Mutation

D70E

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Human Parkin (1-465)

Plasmid

#110758

Purpose

Bacterial expression of human Parkin

Depositor

David Komander

Article

Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

Insert

PRKN (PRKN Human)

Use

Tags

N-His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Smad3

Plasmid

#12630

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
KLC2

Plasmid

#40735

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KLC2 (KLC2 Human)

Use

Tags

6xHis TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pET mCerulean LIC cloning vector (H6-mCerulean)

Plasmid

#29726

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

Biotin - His6 - TEV and mCerulean

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pAJM.474

Plasmid

#108526

Purpose

LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LuxR + PLuxB-YFP

Use

Tags

Expression

Bacterial

Mutation

VanR: T49I A111V P179S

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2A

Plasmid

#15780

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2A (UBE2A Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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