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Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
UseTagsHA-6HIS and MKIEEExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-std
Plasmid#109417PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAK405
Plasmid#37114DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+FFlucRT
Plasmid#49997PurposeIntegrating vector with an improved Red-shifted Thermostable Firefly LuciferaseDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13 from M. marinumAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTNSQCas
Plasmid#219434PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInsertTnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-LoxP
Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIBR4-Cre
Plasmid#219435PurposeTheophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinaseDepositorInsertintron 4-Cre
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-entry(BsaI)
Plasmid#190274PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(BsaI)
UseTagsExpressionBacterialMutationnoPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pReg-RamR
Plasmid#190610PurposeExpresses the RamR transcription factor in E. coliDepositorInsertRamR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAD29_hIRG1_4-461_pvp008
Plasmid#124843PurposeExpresses human CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 462-end.PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJDC60
Plasmid#157780PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterL5Available sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548DepositorInsertHA-hM4D
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6Ă—His-Tag
GyrA-CBD-10xHis-Tag
UseTags10xHis and 6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pML597
Plasmid#32235DepositorInsertmflpe
UseTagsExpressionBacterialMutationM.tuberculosis codon optimized flpe genePromoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSELIS-RamR
Plasmid#202631PurposeEnables dual growth and fluorescence based enrichment of RamR biosensorsDepositorInsertSh ble
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLambda promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCoofy2
Plasmid#43981DepositorTypeEmpty backboneUseTagsTrx-His6ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZE21-GFPaav
Plasmid#26643DepositorInsertpZE21-GFPaav
UseTagsGFPaavExpressionBacterialMutationnaPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only