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Plasmid#105974Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesionsDepositorInsertPaxillin
UseTagsmKateExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
819 pGEX2T PTEN C124S
Plasmid#10745DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationC124SPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMRE137
Plasmid#118491Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p4X
Plasmid#75289PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneUseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pARC8-LambdaRedBeta-EcSSB-EcMutLE32K
Plasmid#162576PurposeArabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.DepositorInsertsLambda Red-Beta
E. coli SSB
EcMutLE32K
UseTagsExpressionBacterialMutationE32K mutated to give dominant negative phenotypePromoterpBADAvailable sinceMay 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pQLinkHD
Plasmid#13668DepositorInsertGateway cassette
UseCo-expressionTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-mRFP1-P2A-Puro
Plasmid#160228PurposeExpresses mRFP in mammalian cellsDepositorInsertmRFP1-P2A-Puro
UseRetroviralTagsExpressionBacterial and MammalianMutationPromoterLTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest
Plasmid#79784PurposeExpression plasmid - BsmBI entry sitesDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterpTrcAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
UseTagsN-terminal Ssp intein (His-tagged)ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZE31-DsRed-ms2x2
Plasmid#26248DepositorInsertms2 binding sites (x2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFNF
Plasmid#22687DepositorInsertNeo*
UseMouse TargetingTagsExpressionBacterial and MammalianMutationFRT-pPGK-pEM7-Neo*-bpA-FRTPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only