-
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HiGlpG
Plasmid#158538PurposeBacterial expression of rhomboid protease HiGlpGDepositorInsertHiGlpG
UseTagsGST tagExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGT-Kh2
Plasmid#122587PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pMBP-HIS2-Snf7
Plasmid#21492DepositorInsertSNF7 (SNF7 Budding Yeast)
UseTags6x His and MBPExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSK+KanaRpsL
Plasmid#20871DepositorInsertKanamycinResistance-RpsL
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH181.3
Plasmid#135076PurposeCoryne. glutamicum HA production plasmidDepositorInserthasA-hasC
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-hcGAS(157-522)
Plasmid#108676PurposeFor expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522DepositorInsertcGAS amino acid 157-522 (CGAS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acid 157-522PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-hnRNP-H
Plasmid#23020DepositorInserthnRNP-H (HNRNPH1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
UseTagsTRX-HisExpressionBacterialMutationPromoterT7Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-LRPAP1
Plasmid#31327DepositorInsertlow density lipoprotein receptor-related protein associated (LRPAP1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-3a_mb2m
Plasmid#211368PurposeExpresses murine b2mDepositorInsertb2m (B2m Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Vch)-entry(BsaI)
Plasmid#190273PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)
UseTagsExpressionBacterialMutationnoPromoterAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTG131
Plasmid#198640PurposeThermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoterDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterpPhlFAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD451-pA-APEX2
Plasmid#157863PurposeBacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labelingDepositorInsertpA-APEX2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only