-
Plasmid#14034DepositorInsertAzurite
UseYeast/bacteria optimized codonsTags6xHisExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYTK077
Plasmid#65184PurposeEncodes KanamycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertKanamycinR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KIRIN1
Plasmid#107414PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1DepositorInsertKIRIN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD279.8
Plasmid#173171PurposeEncodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporterDepositorInsertsSO_4388(REC)-PsdR(DBD) 137
sfGFP
UseTagsExpressionBacterialMutationPromoterJ23108 and PpsdA110Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pET24a-VHH-std
Plasmid#109417PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIA1385
Plasmid#166862PurposeCodon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.DepositorInsertSARS-COV-2 Nsp12 (ORF1ab Synthetic)
UseTagsHis-SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorInsert–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
UseTagsNOExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA
Plasmid#176742PurposeExpresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytesDepositorInsertAqp4-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.D2-NP
Plasmid#134366PurposeNanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.DepositorInsertD2-NP (DRD2 Human)
UseTagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYTK033
Plasmid#65140PurposeEncodes Venus as a Type 3 part to be used in the Dueber YTK systemDepositorInsertVenus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorInsertp62 FL (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)
Plasmid#160736PurposeSingle-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1029
Plasmid#22066DepositorTypeEmpty backboneUseTagsT25-FlagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cra-GFP
Plasmid#220151PurposeTo track the natural Cra promoter's transcriptional dynamicsDepositorInsertCra promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRP-GFP
Plasmid#220152PurposeTo track the natural CRP promoter's transcriptional dynamicsDepositorInsertCRP promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fis-GFP
Plasmid#220153PurposeTo track the natural Fis promoter's transcriptional dynamicsDepositorInsertFis promoter only
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only