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Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
UseTagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM597
Plasmid#68886PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-jAspSnFR3
Plasmid#203458PurposejAspSnFR3 in T7 bacterial expression vectorDepositorInsertjAspSnFR3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Vps2
Plasmid#21494DepositorInsertVPS2 (DID4 Budding Yeast)
UseTags6x His and MBPExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-14-3-3 beta GST
Plasmid#13276DepositorInsert14-3-3 beta (YWHAB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
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MBP-C5E-6XTUBE
Plasmid#217628PurposeBacterial expression of MBP tagged 6XTUBEDepositorInsert6XTUBE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hela kinase-dead MLCK-GFP
Plasmid#46317DepositorInsertHeLa long myosin light chain kinase-dead (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationE1626KPromoterCMVAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCST-avGFP
Plasmid#129508PurposeExpresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)DepositorInsertavGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-C
Plasmid#185481PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneUseTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFG036 + pFG051
Plasmid#137997PurposeTransposon mutagenesis plasmid and the cI repressor expression plasmidDepositorInsertTn5 tnp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SuperClomeleon
Plasmid#203724PurposeAn improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chlorideDepositorInsertCerulean linked topaz fluorescent protein
UseTagsExpressionBacterial and MammalianMutationCerulean, modified Topaz Fluorescent Protein with…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAL-hCul1-NTD
Plasmid#29518DepositorInsertCUL1 (CUL1 Human)
UseTagsThrombin Cleavage SiteExpressionBacterialMutationN-terminal 415 aaPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSC101_TIMER
Plasmid#103057Purposelow copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SUV420H2
Plasmid#32864PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSUV420H2 (KMT5C Human)
UseTagsHis tag (with TEV protease)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrc99S-ssDsbA-CRM197-4xDQNAT
Plasmid#128403PurposePeriplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationInactivating G52E mutationPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -