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12.4kbVillin-ΔATG

Plasmid

#19358

Depositor

Deborah Gumucio

Article

Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

Insert

12.4kb Villin promoter

Use

Tags

Expression

Bacterial

Mutation

No ATG - transgenes cloned downstream of the vill…

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pTriEx4-H6-FGAm (FlincG3)

Plasmid

#49202

Purpose

Fluorescent reporter for cGMP

Depositor

John Garthwaite

Article

Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

Insert

FlincG3

Use

Tags

His tag + other tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Met to Lys in cpEGFP region similar to CGaMP3

Promoter

CMV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pTE1062

Plasmid

#172356

Purpose

Detection of Streptomyces coelicolor butyrolactones (SCB) by kanamycin bioassay. Streptomyces genomic integrative vector.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al Methods Mol Biol. 2018;1673:117-128. doi

Inserts

scbR (SCO6265 )
kasOp

Use

Synthetic Biology; Integrative into streptomyces …

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX 6P1 CDK2 (552)

Plasmid

#61845

Purpose

bacterial expression of GST CDK2 WT

Depositor

Jonathon Pines

Insert

CDK2 (CDK2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pKB230

Plasmid

#170017

Purpose

Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Protegrin-1

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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spCas9-NLS-MAV

Plasmid

#124212

Purpose

SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

Depositor

Uri David Akavia

Article

Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

Insert

cas9

Use

CRISPR

Tags

His6, MBP, TEV Cleavage site, and two copies of S…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUCXMG

Plasmid

#204409

Purpose

Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

Depositor

David Ackerley

Article

Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116954

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET_RFP-SZ2

Plasmid

#124933

Purpose

Expression of RFP-SZ2

Depositor

Benjamin Schuster, Matthew Good, Daniel Hammer

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

SZ2 domain

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-5u

Plasmid

#13554

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi S52A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

S52A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pUCP20T-tdTomato

Plasmid

#78469

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHMattR3-attR4-GFP

Plasmid

#196247

Purpose

pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA (B. megaterium)

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoAp-GRFT

Plasmid

#158747

Purpose

low phosphate phoAp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoAp Griffithsin

Use

Tags

Expression

Bacterial

Mutation

Promoter

phoA

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Use

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

Plasmid

#105234

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mu-FLT3Lv1-pTRIP

Plasmid

#163519

Purpose

Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

Depositor

Thomas Weber

Insert

murine-FLT3L variant 1 (Flt3l Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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