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Plasmid#79057PurposeA C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1DepositorInsertC-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
UseLentiviralTagsFLAGExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx1
Plasmid#51558PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coliDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDest-544
Plasmid#11519DepositorTypeEmpty backboneUseGateway destination vectorTagsHis6-NusAExpressionBacterialMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCambia1300-PacI
Plasmid#44183DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUS252
Plasmid#127674PurposeExpresses fuGFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFP to place in other plasmid backbonesDepositorInsertFree Use GFP (fuGFP)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJune 24, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF-mcherry1
Plasmid#87144PurposeConstitutive E. coli mcherry ExpressionDepositorInsertmcherry
UseTagsExpressionBacterialMutationPromoterConstitutive proDAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
ScSpe1 pET-20b(+)
Plasmid#117145PurposeExpresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21DepositorInsertSPE1
UseTagsHistidineExpressionBacterialMutationPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS30: pTarget(PmcCAST)
Plasmid#168163PurposeTarget plasmid containing PmcPSP1 and attachment site for PmcCAST.DepositorInsertsPAM for PmcCAST + PmcPSP1
tRNA-Val
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-moxGFP2
Plasmid#218957PurposeExpresses proinsulin fused to moxGFP2 in bacteriaDepositorInsertproinsulin-moxGFP2
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
proinsulin-mGold
Plasmid#218953PurposeExpresses proinsulin fused to mGold in bacteriaDepositorInsertproinsulin-mGold
UseTagsproinsulinExpressionBacterialMutationPromoterT5Available sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-mScarlet-I
Plasmid#209993PurposeContains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmidsDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dox-Akt
Plasmid#207328PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)DepositorInsertrac protein kinase-alpha (AKT1 Human)
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 129 - 480PromoterTet Response ElementAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99F
Plasmid#149439PurposeExpression of MIF Y99F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD185
Plasmid#113317PurposedCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
UseTags6xHisExpressionBacterialMutationPromoteraraBAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only