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Addgene
Showing: 2861 - 2880 of 28135 results

  3. pBAD-MBP-His-SFP

    Plasmid
    #141141
    Purpose
    Expression of 4'-phosphopantetheinyl transferase gene
    Depositor
    New England Biolabs
    Insert
    4'-phosphopantetheinyl transferase
    Use
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141141-plasmid-map-sequence-id-298488

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  5. pET21b-Casp1-His

    Plasmid
    #11809
    Depositor
    Guy Salvesen
    Insert
    Caspase-1 (CASP1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only
    11809-plasmid-map-sequence-id-205971

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  6. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    119945-plasmid-map-sequence-id-237587

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  10. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  11. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

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  16. T5-sgRNA

    Plasmid
    #113655
    Purpose
    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.
    Depositor
    Jeffrey Barrick
    Article
    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531
    Insert
    promoter T5 and sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113655-plasmid-map-sequence-id-218814

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  17. SMS

    Plasmid
    #25234
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMS_01
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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Showing: 2861 - 2880 of 28135 results