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Plasmid#85502PurposePlasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertp-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32T, E107T, D158P, I159L, L162APromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCPP3373
Plasmid#128718PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopQ1-1
UseTagsExpressionBacterialMutationSynonymous point mutation in the wobble position…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4b
Plasmid#51073PurposePhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)DepositorInsertmEos4b
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG261-His-greenFAST
Plasmid#160349PurposeExpresses His-tagged greenFAST in bacteriaDepositorInsertHis-greenFAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorInsertgfpmut2 (NEWENTRY Synthetic)
UseTagsExpressionBacterialMutationPromoterPxyl(s)Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23119Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX iLOV
Plasmid#26587DepositorInsertiLOV
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Cterm_His
Plasmid#220487Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Human_SAT1_Nterm_His
Plasmid#220482Purposeheterologous expression of SSAT1 for protein purificationDepositorInsertSSAT1 (SAT1 Human)
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_speG_Cterm_His
Plasmid#220483Purposeheterologous expression for protein purificationDepositorInsertspeG
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2n-6xHis
Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseTags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
ISA431: aTc-MP6
Plasmid#189935PurposeMutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanRDepositorInsertdanQ926, dam, seqA, emrR, ugi and cda1
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
KG#205
Plasmid#110881PurposeExpresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationchanged Asp 448 to AsnPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmVirD2-GFP
Plasmid#90315PurposemVirD2-GFP expression plasmidDepositorInsertA. tumefaciens VirD2 from aa 1 to 204 - GFP
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7/T5 IPTG inducibleAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109Hg-gfp
Plasmid#123148PurposeA mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerTAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZ853
Plasmid#208115PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSDDepositorInsertmNeonGreen-HPRT1-BSD
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBCPB+
Plasmid#18940DepositorInsertattP, lacZ, attB
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only