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Plasmid#198356PurposeExpress M.CviPII (NDel15) in bacterial cells.DepositorInsertM.CviPII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNW-sfGFP(SPS6)
Plasmid#133814PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPptaAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIB184 (EM)
Plasmid#90194PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_8-eGFP
Plasmid#107411PurposeThis plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPthrC3_8Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGSTag
Plasmid#21877DepositorTypeEmpty backboneUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3a
Plasmid#203689PurposeExpresses N8C_Fab3aDepositorInsertN8C_Fab3a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P1
Plasmid#27366DepositorInsertPmyc1tetO
UseGateway donor vectorTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWZ403
Plasmid#208113PurposemSwAP-In marker cassette 1 with mScarlet-PuroTKDepositorInsertmScarlet-PuroTK
UseTagsP2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))
Plasmid#218508Purposeintegrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertmNeongreen
UseTagsmodified ssrA degradation tagExpressionBacterialMutationdeletion of MCS containing Plac_lacZa, introducti…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSKrnaSeph
Plasmid#67506PurposeExpress the tRNALys scaffold with the sephadex aptamer tagDepositorInsertmodified tRNALys with sephadex aptamer
UseTagsExpressionBacterialMutationPromoterlppAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-hAos1
Plasmid#53135PurposeE.coli expression of full length human Aos1 (SAE1) with Nterminal His-tagDepositorInsertAos1 (SAE1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KC (KanR, PhiC31)
Plasmid#45982DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delBRCT
Plasmid#173938PurposeBacterial expression of PARP1 lacking BRCT domainDepositorInsertPARP1delBRCT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 374-520; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only