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Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
UseTagsP2A-mCherryExpressionBacterialMutationPromoterno promoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHTganA-Cpf1
Plasmid#158647PurposeConstitutive transcription of FnCpf1 and ganA crRNADepositorInsertCpf1, ganA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterP43Available sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-Cas12m
Plasmid#192274PurposeEncodes MmCas12m under a constitutive promoterDepositorInsertMmCas12
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINM
Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
UseTagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMBI
Plasmid#17816DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Nanog
Plasmid#16351DepositorInsertHomo sapiens Nanog homeobox (NANOG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKD4_mCherry
Plasmid#187386PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRTDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINJ
Plasmid#26045DepositorInsertHisGST3C-lacZ
UseTagsMAHHHHHH_GST_LEVLFQGTExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
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RALGDS
Plasmid#25331PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRALGDS:N793-F914 (RALGDS Human)
UseTagsHisExpressionBacterialMutationContains amino acids N793-F914PromoterAvailable sinceSept. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-26b-Nb127D01-HA-His
Plasmid#171566PurposeBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)DepositorInsertNb127D01-HA-His (CXCR2 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_ENO1-Halo
Plasmid#175330PurposeProduction of HaloTag fusion proteinDepositorInsertENO1 (ENO1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-coSCFP3A
Plasmid#104015PurposePlasmid encoding coSCFP3ADepositorInsertcoSCFP3A
UseLow copyTagsExpressionBacterialMutationSame protein sequence as SCFP3A (codon optimized …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
Plasmid#160540PurposeExpresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMPTY::sgRNA2
Plasmid#165459PurposeEscherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteriaDepositorInsertsgRNA2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterSP01Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-cdaR
Plasmid#62557PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertsbeta lactamase
sfGFP
CdaR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only