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Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET28a_DmKHC[1-421]-1xGFP-6xHis
Plasmid#196972PurposeBacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTags6xHis and GFPExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-HyPer3
Plasmid#42132PurposeGenetically encoded H2O2 probe for ratiometric and fluorescence lifetime imagingDepositorInsertHYPER 3
UseTags6HisExpressionBacterialMutationPromoterT5Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2844
Plasmid#160675PurposeMobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0679
Plasmid#149689PurposeBacterial expression of SENP_EuH proteaseDepositorInsertSENP_EuH protease
UseTagsHis14-tag and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1
Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC8 human IFN-gamma
Plasmid#17600DepositorInsertinterferon-gamma cDNA (IFNG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRE118
Plasmid#43830DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1TagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD/myc-his A Rv0712 (FGE)
Plasmid#16132PurposeBacterial expression of Mycobacterium tuberculosis FGEDepositorInsertFormylglycine-generating enzyme
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
UseTagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Lac-dCas9 (Ptac)
Plasmid#105234PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescensDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599APromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
NylB'-SCY_pET21b
Plasmid#215417PurposeExpression construct for NylB'-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB'-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL3-pCMV7.1
Plasmid#155021PurposeN-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vectorDepositorInsertCullin 3 (CUL3 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT5Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD-eGFP
Plasmid#112918PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPbadAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only