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Plasmid#119969PurposeBacterial expression for structure determinationDepositorInsertTET3 (TET3 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 48-98PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Pthl
Plasmid#90485PurposePlasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lbpro (29-195)
Plasmid#110759PurposeBacterial expression of LbproDepositorInsertLb pro
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM5082
Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
UseTagsExpressionBacterialMutationAraC: L133F E165L E169G C280LPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF48 HLA-A*11:01
Plasmid#180449PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*11:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF6 HLA-A*24:02
Plasmid#180445PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*24:02
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYX-C-mScarlet-Zeo-TSC22D2
Plasmid#222670Purposeendogenously tag TSC22D2 with C-terminal mScarletDepositorInsertTSC22D2 (TSC22D2 Human)
UseTagsmScarletExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-TSC22D4
Plasmid#222671Purposeendogenously tag TSC22D4 with N-terminal mNeonDepositorInsertTSC22D4 (TSC22D4 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN6
Plasmid#180287PurposeHygR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - HygR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCri_L706-antiSH2-OptoMB
Plasmid#159935PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CYP51A1 (3I3K)
Plasmid#25161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCYP51A1_cw_09 (CYP51A1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 54-502PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits