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Plasmid#89960PurposeContains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.DepositorInsertempty gRNA
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD IFP1.4
Plasmid#91775PurposeBacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)DepositorInsertInfrared Fluorescent Protein 1.4 + Heme oxygenase-1
UseTagsHexa-Histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
UseTags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612APromoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTXTL-T7max-RLuc
Plasmid#188312PurposeExpression of Renilla luciferase via a T7 promoter in TXTLDepositorInsertRenilla luciferase
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-E2Crimson
Plasmid#78473PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp3 C163A-His
Plasmid#11822DepositorInsertCaspase 3 catalytic mutant (CASP3 Human)
UseTags6xHisExpressionBacterialMutationC163APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15-UbE2D1
Plasmid#15782DepositorInsertUbiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
UseTags6x His, T7 tag, and Xpress tag (enterokinase clea…ExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S14
Plasmid#128596PurposeExpresses tagless 30S ribosomal subunit protein S14 in E.coliDepositorInsert30S ribosomal subunit protein S14 (rpsN E.coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-CLIP
Plasmid#127180PurposeBacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tagDepositorInsertSNAP-CLIP
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCL151
Plasmid#134455PurposeTetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.DepositorInsertlinker-mScarlet-I for C-terminal fusion
UseTagsExpressionBacterialMutationPromoterplacAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCL147
Plasmid#134454PurposeTetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.DepositorInsertlinker-mScarlet-I for C-terminal fusion
UseTagsExpressionBacterialMutationPromoterplacAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only