-
-
pET15-UbE2D2
Plasmid#15783DepositorInsertUbiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorInsertLC3B (MAP1LC3B Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pBSV2G_PflaB-mCherryBb
Plasmid#118230PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR-Cas9ΔyqhD-HA500
Plasmid#220369PurposeA single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440DepositorInsertsSpCas9
sgRNA for yqhD in P. putida KT2440
UseCRISPR; Broad host rangeTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
UseTags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST PTBP1 isoform 4
Plasmid#108594PurposeRecombinant GST tagged protein productionDepositorInsertPTBP1 isoform 4 (PTBP1 Human)
UseTagsGSTExpressionBacterialMutationisoform 4 (isoform a)PromotertacAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
K560GFP_17NL
Plasmid#129767PurposeExpresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assaysDepositorInsertKHC (Khc Fly)
UseTagsGFP and His6ExpressionBacterialMutationDAL insert before A345PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW019-2
Plasmid#85614PurposeInducible dCas9 integration vector for Bacillus subtilisDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterPxylA (B. megaterium)Available sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-smURFP-RBS-HO-1
Plasmid#80341PurposeExpresses smURFP and Heme Oxygenase-1 in bacterial cells.DepositorInsertssmURFP
HO-1
UseTags6 Histidine TagExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMG-RIS
Plasmid#225132PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HisMS2_VLP_FluA
Plasmid#175955PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]DepositorInsertM gene
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
mCitrine-pBAD
Plasmid#54723PurposeLocalization: Protein Expression Vector , Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTags6xHis and mCitrineExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET32a-hEGF
Plasmid#199231PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpidermal growth factor, partial (EGF Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only
Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
How are we doing? Share feedback for a chance to win $50
Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more
Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more
