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Plasmid#88871Purposeinducible expression of GST-DDX5 in bacteriaDepositorInsertDDX5 (DDX5 Human)
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-MT-P2A
Plasmid#214896PurposeExpresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sitesDepositorInsertSPLICSFAST-LDh-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
UseTags3xFlag and intein-CBDExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMK803
Plasmid#207138PurposeAssembled Tn10 with CP25 promoter driving gfpDepositorInsertTn10-CP25-GFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-Violacein-pT7native
Plasmid#200002PurposeViolacein pathway. pT7-native CDS sequenceDepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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tracrRNA(scr)-CJ8421_04975 mRNA
Plasmid#199718PurposeExpresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequenceDepositorInsertCJ8421_04975 mRNA and scrambled tracrRNA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSiM1
Plasmid#199380PurposeEncodes dual cistronic membrane localized GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6s::ras-2CAAX::SL2::mScarlet-I::ras-2CAAX
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-acrAB-sfGFP
Plasmid#195516PurposeInducible expression vector for transcriptionally fused acrAB and sfGFP.DepositorInsertacrAB
UseSynthetic BiologyTagssfgfpExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorInsertElo3 (ELO3 Budding Yeast)
UseTagsGFP11 and HDELExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_M337P
Plasmid#98675PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with M337PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationM337PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A326P
Plasmid#98672PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A326PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA326PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
UseTagsExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFKM1
Plasmid#64950Purposesource of KmR FRT cassetteDepositorInsertFRT-KmR-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMV361_MjTyrRS
Plasmid#193250PurposeExpresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)DepositorInsertMjTyrRS
UseTagsExpressionBacterialMutationPromoterhsp60Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterermEp* promoter from S. erythraeaAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP3665
Plasmid#222351PurposeExpression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRA_Hormone-recep
Plasmid#109908PurposeProtein expression and purification of ESRRA_Hormone-recepDepositorInsertESRRA_Hormone-recep (ESRRA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2H12
Plasmid#221142PurposecdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204)DepositorInsertcdGreen
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneUseTagsluxCDABEExpressionBacterialMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only