Addgene

New search

Keep filters

28,130 results

Showing: 2261 - 2280 of 28130 results

Results per page:

Sort results by:

RepairTemplate_NIPBL_mEGFP_mAID

Plasmid

#217664

Purpose

Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

NIPBL_mEGFP_mAID (NIPBL Human)

Use

Tags

mAID and meGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RHOAA-c033

Plasmid

#205116

Purpose

RHOA protein expression

Depositor

Paul Brennan

Insert

RHOA (RHOA Human)

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

WT

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLux and pTet

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Use

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SENP1 (catalytic domain)

Plasmid

#16356

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP1 catalytic domain (SENP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 419 to 644 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pKOV

Plasmid

#25769

Depositor

George Church

Article

Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Lac-I-SceI-Tet

Plasmid

#17655

Depositor

Tom Misteli

Article

Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

Insert

lac I-SceI tet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2008

Availability

Academic Institutions and Nonprofits only
pGEX 6P1 CDK2 (552)

Plasmid

#61845

Purpose

bacterial expression of GST CDK2 WT

Depositor

Jonathon Pines

Insert

CDK2 (CDK2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pSCB2-sgRNA

Plasmid

#129463

Purpose

Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

pgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 (SpeI)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNSQCas

Plasmid

#219434

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonor-LoxP

Plasmid

#219436

Purpose

CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

two transposon ends, LoxP-terminator-LoxP gene cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCasTns(Ptr)-entry(BsaI)

Plasmid

#190274

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

no

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Skp

Plasmid

#213139

Purpose

Expresses chaperone protein SKP

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SKP (21-161) (skp E.coli)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6

Plasmid

#49795

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge