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EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
UseTagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ854
Plasmid#208116PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-NeoDepositorInsertmNeonGreen-HPRT1-Neo
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ852
Plasmid#208114PurposemSwAP-In marker cassette 1 with mScarlet-HygTKDepositorInsertmScarlet-HygTK
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ699
Plasmid#208117PurposePayload yeast assembly vector with TK-mActb on backboneDepositorTypeEmpty backboneUseSynthetic Biology; Copy number inducibleTagsExpressionBacterial, Mammalian, and Y…MutationPromoterHuman PGK1 for thymidine kinaseAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2E2
Plasmid#15788DepositorInsertUbiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSL1425 (pSPIN, pCDF backbone)
Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
GSK3 beta pGEX
Plasmid#15898DepositorInsertGSK3 beta (GSK3B Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCpf1
Plasmid#122185PurposeBacterial genome editing plasmid with kanamycin resistance markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry
Plasmid#182390PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
OmpA-mCherry
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12.D11
Plasmid#221145PurposeHis6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)DepositorInsertcdGreen2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake1
Plasmid#139340PurposeExpresses Curlcake1 (CC1) in E.coliDepositorInsertCurlcake1 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake2
Plasmid#139341PurposeExpresses Curlcake2 (CC2) in E.coliDepositorInsertCurlcake2 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Curlcake4
Plasmid#139343PurposeExpresses Curlcake4 (CC4) in E.coliDepositorInsertCurlcake4 with T7 promoter
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-MYCN
Plasmid#104542PurposePiggyBac vector encoding doxycycline-inducible human MYCNDepositorInsertMYCN proto-oncogene, bHLH transcription factor (MYCN Human)
UseTagsExpressionBacterial and MammalianMutationPromoterTRE3GAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA1
Plasmid#85709PurposeThis plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK1
Plasmid#53159PurposeExpresses GST-HA tagged human MEK1 from bacterial expression vectorDepositorInsertMEK1 (MAP2K1 Human)
UseTagsGST and HAExpressionBacterialMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only
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