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Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorInsertHis-Tev-UbE2D3 (UBE2D3 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorUseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA707
Plasmid#186238PurposeKanR-BBR1 entry vector for HR donorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144A (16-228)
Plasmid#110752PurposeBacterial Expression of RNF144A RBR domainDepositorInsertRNF144A (RNF144A Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Bst-LF
Plasmid#145799PurposePlasmid for the aTc-inducible expression of the large-fragment of Bst DNAPDepositorInsertBst-LF
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad33_ISYmu1_reRNA
Plasmid#205358PurposeBacterial expression of ISYmu1 TnpB and its reRNADepositorInsertISYmu1 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET MBP His6 LIC cloning vector (2Cc-T)
Plasmid#37237DepositorTypeEmpty backboneUseTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 UBXN2A
Plasmid#113505PurposeBacterial expression of GST-tagged UBXN2ADepositorInsertUBXN2A (UBXN2A Human)
UseTagsGST-Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-CD63
Plasmid#194989Purposeoverexpress CD63DepositorInsertGFP-CD63
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
5TTW (EED)
Plasmid#101254PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEED (EED Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWVR5
Plasmid#167509PurposeBroad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-MH6-Bsu
Plasmid#163911PurposeExpresses Bsu large fragment for affinity purificationDepositorInsertBsu polymerase
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLO-Pink-SHE
Plasmid#220781PurposeExpresses a raspberry chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)DepositorInsertraspberry chromoprotein (mRFP1)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA8
Plasmid#177661PurposeRecombinant protein synthesis of human HSPA8DepositorInsertHSPA8 (HSPA8 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
UseTagsExpressionBacterialMutationPromoterParaAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
UseTagsExpressionBacterialMutationPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only