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Showing: 2021 - 2040 of 28130 results

  1. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  5. roGFP_iE_C147S_pQE30

    Plasmid
    #48631
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, C147S
    Promoter
    Available since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48631-plasmid-map-sequence-id-70379

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  6. pBlueKan+cysMPro

    Plasmid
    #187879
    Purpose
    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    CysM
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187879-plasmid-map-sequence-id-368417

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  9. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  13. AK2

    Plasmid
    #38829
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AK2 (AK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid
    #195741
    Purpose
    Base editing for making a pre-stop codon in mmoX using phenol inducible system
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195741-plasmid-map-sequence-id-386423

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Showing: 2021 - 2040 of 28130 results