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pUS252y

Plasmid

#191834

Purpose

Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.

Depositor

Nicholas Coleman

Insert

fuYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Francesca Storici, Michael Resnick

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-D4

Plasmid

#37473

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D4 second generation cameleon (calcium sensor)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ara

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pL0-MtPT4Pro

Plasmid

#159414

Purpose

Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

MtPT4 Promoter

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-MtBCP1Pro

Plasmid

#159415

Purpose

Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

MtBCP1 Promoter

Use

Puc19-derived

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
MBP-mmA1

Plasmid

#61090

Purpose

expression of fusion protein: MBP-hnRNP A1

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

hnRNP A1 (Hnrnpa1 Mouse)

Use

Tags

H6_MBP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pET28-MBP-TEV-AICD

Plasmid

#69927

Purpose

bacterial expression of MBP-TEV-AICD

Depositor

Thomas Wassmer, Zita Balklava

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

47 aa long

Promoter

T7

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels

Plasmid

#204801

Purpose

Bacterial expresison of Enterovirus D68 2A protease

Depositor

ASAP Discovery, Frank von Delft

Insert

D68 2A protease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pYZ_3WJrep_N24_switch

Plasmid

#132725

Purpose

T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N24_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Article

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Use

Tags

NT* solubility tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLG011 (DRT type 3)

Plasmid

#157889

Purpose

Expresses the DRT type 3 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 3 (RT + RT)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLG009 (DRT type 1)

Plasmid

#157887

Purpose

Expresses the DRT type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 1 (RT-nitrilase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLG003 (Druantia type I)

Plasmid

#157881

Purpose

Expresses the Druantia type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Druantia type I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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