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FR-E01

Bacterial Strain

#118727

Purpose

Expression of dCas9 gene under the control of a Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Bacterial Resistance

None

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
CC1215

Bacterial Strain

#79062

Purpose

BL21(DE3)-based E. coli purine auxotrophic host strain used for the preparation of PurE proteins in a purE background.

Depositor

T. J. Kappock

Bacterial Resistance

Kanamycin

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only
E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt

Bacterial Strain

#45473

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
MM39

Bacterial Strain

#42894

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Bacterial Resistance

Streptomycin

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
JM107

Bacterial Strain

#49759

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM109

Bacterial Strain

#49761

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM106

Bacterial Strain

#49757

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM110

Bacterial Strain

#49763

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

Streptomycin

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt lambda pir

Bacterial Strain

#45472

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
OpLac2-delta-6

Bacterial Strain

#52700

Purpose

OpLac2 without the first 6 nucleotides of lacZ

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
OpLac1-delta-6

Bacterial Strain

#52698

Purpose

OpLac1 without the first 6 nucleotides of lacZ

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
Jun lab tunable CRISPRi strain

Bacterial Strain

#86400

Purpose

Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

Depositor

Suckjoon Jun

Article

Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

Bacterial Resistance

Spectinomycin and hygromycin

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
BW-Para

Bacterial Strain

#172602

Purpose

The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Bacterial Resistance

None

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only
KTD101(DE3)

Bacterial Strain

#138651

Purpose

KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Bacterial Resistance

None

Species

Escherichia coli

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only
HL 1216

Bacterial Strain

#52938

Purpose

MG 1655 + lacIq inserted into the chromosome at the intS site + ΔmicC + ΔaraC_KanR

Depositor

Han Lim

Article

Hussein et al Nucleic Acids Res. 2012 May 22.

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
HL 1254

Bacterial Strain

#52939

Purpose

MG 1655 + lacIq inserted into the chromosome at the intS site + ΔdsrA + ΔaraC_KanR

Depositor

Han Lim

Article

Hussein et al Nucleic Acids Res. 2012 May 22.

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 37 results

Results per page:

Sort results by:

FR-E01

C321.∆A.opt

CC1215

E. coli MEV20

E. coli MEV15

HB101 endA::frt

MM39

JM107

JM109

JM106

JM110

HB101 endA::frt lambda pir

OpLac2-delta-6

OpLac1-delta-6

rEcoli XpS

Jun lab tunable CRISPRi strain

BW-Para

KTD101(DE3)

HL 1216

HL 1254

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