Addgene

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C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
delta guaB

Bacterial Strain

#113653

Purpose

Base strain that the pDCAF plasmids (Addgene #113646-113652) must be in.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Bacterial Resistance

Kanamycin

Available since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits
E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only
39R861+

Bacterial Strain

#119737

Purpose

Six stable plasmids can be used as size standards

Depositor

Ruth Hall

Article

Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

Bacterial Resistance

None

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only
SJ358

Bacterial Strain

#67755

Purpose

a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

Depositor

Suckjoon Jun

Article

Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

Bacterial Resistance

None

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
sAJM.1506

Bacterial Strain

#108254

Purpose

Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only
sAJM.1505

Bacterial Strain

#108253

Purpose

Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
sAJM.1504

Bacterial Strain

#108251

Purpose

Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
RF17

Bacterial Strain

#102801

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
EcNR2.dnaG.Q576A

Bacterial Strain

#41700

Depositor

George Church

Article

Lajoie et al Nucleic Acids Res. 2012 Aug 16.

Bacterial Resistance

Ampicillin

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
EcNR2 Strain

Bacterial Strain

#26931

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
Nuc5-.dnaG.Q576A.tolC-

Bacterial Strain

#41699

Depositor

George Church

Article

Lajoie et al Nucleic Acids Res. 2012 Aug 16.

Bacterial Resistance

Ampicillin

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
EcNR1 Strain

Bacterial Strain

#26930

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
BA14

Bacterial Strain

#186997

Purpose

Deletion of the nuo operon

Depositor

Steven Vik

Article

Amarneh et al Biochim Biophys Acta. 2006 Dec;1757(12):

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only
∆cosσε ∆HG

Bacterial Strain

#197132

Purpose

Deficient in the packaging cos site and encoding rhamnose-inducible P4 delta and epsilon genes. It also has tail fiber (H) and its chaperone (G) deleted.

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Bacterial Resistance

Hygromycin

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
rEc.13.delA

Bacterial Strain

#69495

Purpose

Recoded strain from MG1655, prfA deleted

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 129 results

Results per page:

Sort results by:

C321.∆A.opt

delta guaB

E. coli ER1821ΔlacI

39R861+

SJ358

sAJM.1506

sAJM.1505

sAJM.1504

Escherichia coli DH10B-MOB

RF17

EcNR2.dnaG.Q576A

EcNR2 Strain

Nuc5-.dnaG.Q576A.tolC-

EcNR1 Strain

BA14

∆cosσε ∆HG

E. coli K-12 MG1655_rLP5(nth-ydgR)

rEc.13.delA

E. coli K-12 MG1655_fLP4(essQ-cspB)

E. coli K-12 MG1655_rLP6(ygcE-ygcF)

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