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C321.∆A ompT- rne- endA- lon-

Bacterial Strain

#182775

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
MCJ.559

Bacterial Strain

#69493

Purpose

Strain with recoded TAG and genomic deletions for improved incorporation

Depositor

Michael Jewett

Article

Hong et al Chembiochem. 2015 Mar 23;16(5):844-53. d

Bacterial Resistance

Ampicillin

Species

Eschericia coli

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- lon-

Bacterial Strain

#182773

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
rEc.13.delA

Bacterial Strain

#69495

Purpose

Recoded strain from MG1655, prfA deleted

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
rEc.13

Bacterial Strain

#69494

Purpose

Recoded strain from MG1655 with prfA

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
C321.∆A T7RNAP ompT- rne- lon-

Bacterial Strain

#182778

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Jean-Loup Faulon, Chase Beisel

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecB

Bacterial Strain

#176580

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Jean-Loup Faulon, Chase Beisel

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
C-5545 ∆cosδε

Bacterial Strain

#209018

Purpose

Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

Depositor

Alfonso Jaramillo

Article

Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

Bacterial Resistance

Hygromycin

Species

Escherichia coli

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only
Strain CSH100

Bacterial Strain

#21875

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Bacterial Resistance

None

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
EH1

Bacterial Strain

#102804

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF17

Bacterial Strain

#102801

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF18

Bacterial Strain

#102802

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

Showing: 61 - 80 of 83 results

Results per page:

Sort results by:

C321.∆A ompT- rne- endA- lon-

C321.∆A T7RNAP ompT- rne-

MCJ.559

C321.∆A ompT- rne- lon-

rEc.13.delA

rEc.13

E. coli BW25113 ΔtnaA ΔtrpR

C321.∆A T7RNAP ompT- rne- lon-

BL21 ΔrecBCD

BL21 ΔrecB

C-5545 ∆cosδε

Strain CSH100

C321.ΔA.exp

C321.ΔA

C321.deltaA (Isaacs lab)

B95(DE3) ΔA ΔfabR ΔserB

Escherichia coli DH10B-MOB

EH1

RF17

RF18

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