Strain CSH100
(Bacterial strain #21875)

• Depositing Lab: Keith Joung
• Publication: Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Backbone

• Vector backbone: n/a

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): CSH100
• Growth instructions: CSH100 is not resistant to any antibiotics. We recommend that it be streaked out on an M9 minimal plate (with glucose) in order to maintain selection for the F’.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CSH100 bacterial Strain

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

F’ lac proA+proB+(lacIq lacPL8)/ara- ∆(gpt-lac)5

This strain will be shipped as bacteria in an LB stab. Upon receipt, requesting scientists should restreak the strain on an M9 minimal plate. After restreaking on M9 to confirm the presence of the F', scientists can grow the strain in liquid LB.

M9 minimal medium agar plates: To prepare 500 ml, autoclave 439 ml H2O with 7.5 g Bacto-agar and a stir bar. When agar has cooled to approximately 65°C, add 50 ml 10X M9 salts, 1 ml 1 M MgSO4, 10 ml 20% (w/v) glucose and 0.5 ml 100mM CaCl2 and then pour plates. Plates can be stored indefinitely at 4°C in sealed plastic bags. (Alternatively, M9 plates can be purchased from Teknonva: https://www.teknova.com/content/teknova/us/en/products/product-page.html/m1260.html)