Addgene

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AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
BL21ΔABCF

Bacterial Strain

#102270

Purpose

ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

Tetracycline

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
TB204

Bacterial Strain

#230033

Purpose

Fluorescently labelled (monomeric sfGFP) E. coli

Depositor

Calin Guet, Tobias Bergmiller

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Bacterial Resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
BJ5183 cells

Bacterial Strain

#16398

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
V. natriegens NC7

Bacterial Strain

#215356

Purpose

Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

Depositor

Buz Barstow

Bacterial Resistance

None

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial Strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only
DH10B-ALT

Bacterial Strain

#61151

Purpose

DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Bacterial Resistance

None

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
RF15

Bacterial Strain

#102799

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
S2060

Bacterial Strain

#105064

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

Bacterial Resistance

Streptomycin and tetracycline

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only
TB201

Bacterial Strain

#230031

Purpose

Fluorescently labelled (YFP) E. coli

Depositor

Calin Guet, Tobias Bergmiller

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
pRK24

Bacterial Strain

#51950

Purpose

broad-host range natural conjugative plasmid

Depositor

Farren Isaacs

Article

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial Resistance

Tetracycline

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
MG1655 ΔendA ΔrecA

Bacterial Strain

#37853

Depositor

Kristala Prather

Article

Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

Bacterial Resistance

None

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 408 results

Results per page:

Sort results by:

AdEasier-1 cells (strain)

BL21ΔABCF

EC1000

TB204

BL21(DE3)-R3-pRARE2

BJ5183 cells

B-95.ΔAΔfabR

B-95.ΔA

V. natriegens NC7

RF6

BL21(DE3) ΔserC

DH10B-ALT

B95(DE3) ΔA ΔfabR ΔserB

RF15

S2060

TB201

pRK24

RF11

DHIP1

MG1655 ΔendA ΔrecA

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