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B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
BL21ΔABCF

Bacterial Strain

#102270

Purpose

ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

Tetracycline

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
Syn61Δ3(ev5) ΔrecA (ev1)

Bacterial Strain

#189857

Purpose

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

Depositor

George Church

Article

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Bacterial Resistance

Streptomycin

Species

Synthetic

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only
Ochre (rEcΔ2.ΔA.B3.tW*)

Bacterial Strain

#234622

Purpose

Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

Depositor

Farren Isaacs

Article

Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Bacterial Resistance

Hygromycin

Available since

May 16, 2025

Availability

Academic Institutions and Nonprofits only
LC-E75

Bacterial Strain

#115925

Purpose

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only
S1030

Bacterial Strain

#105063

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

Bacterial Resistance

Streptomycin and tetracycline

Available since

March 21, 2018

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Bacterial Resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
C321.ΔA.M9adapted

Bacterial Strain

#98568

Purpose

Recoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotin

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Bacterial Resistance

Ampicillin

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655 RARE

Bacterial Strain

#61440

Purpose

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Depositor

Kristala Prather

Article

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Bacterial Resistance

None

Available since

Feb. 1, 2015

Availability

Academic Institutions and Nonprofits only
AdEasier-1 cells (strain)

Bacterial Strain

#16399

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Ampicillin and streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
BJ5183 cells

Bacterial Strain

#16398

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecBCD

Bacterial Strain

#176581

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Jean-Loup Faulon, Chase Beisel

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 412 results

Results per page:

Sort results by:

B95(DE3) ΔA ΔfabR ΔserB

BL21ΔABCF

Syn61Δ3(ev5) ΔrecA (ev1)

Ochre (rEcΔ2.ΔA.B3.tW*)

LC-E75

S1030

B-95.ΔAΔfabR

C321.ΔA

BL21(DE3)-R3-pRARE2

C321.ΔA.M9adapted

RF11

C321.deltaA (Isaacs lab)

E. coli K-12 MG1655 RARE

AdEasier-1 cells (strain)

EC1000

BJ5183 cells

C321.ΔA.exp

rEcoli XpS

C321.∆A.opt

BL21 ΔrecBCD

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