422 results

1. Ochre (rEcΔ2.ΔA.B3.tW*)

   Bacterial Strain

   #234622

   Purpose

   Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

   Depositor

   Farren Isaacs

   Article

   Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Bacterial Resistance

   Hygromycin

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

2. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

3. JM109

   Bacterial Strain

   #49761

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

4. RF4

   Bacterial Strain

   #62072

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

   Bacterial Resistance

   None

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. E. coli BL21 (DE3) ΔEntD

   Bacterial Strain

   #192874

   Purpose

   Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

   Depositor

   Christian Chalut, Christophe Guilhot

   Article

   Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

6. C321.ΔA.exp

   Bacterial Strain

   #49018

   Purpose

   This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

   Depositor

   George Church

   Article

   Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

   Bacterial Resistance

   Bleocin (zeocin)

   Species

   Escherichia coli

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Δrpi

   Bacterial Strain

   #234837

   Purpose

   E. coli strain that grows on glycerol only when it expresses functional rubisco. Contains carbonic anhydrase.

   Depositor

   David Savage

   Bacterial Resistance

   None

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

8. S1030

   Bacterial Strain

   #105063

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

   Bacterial Resistance

   Streptomycin and tetracycline

   Available since

   March 21, 2018

   Availability

   Academic Institutions and Nonprofits only

9. B95(DE3) ΔA ΔfabR ΔserB

   Bacterial Strain

   #197655

   Purpose

   This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Bacterial Resistance

   None

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

10. AdEasier-1 cells (strain)

    Bacterial Strain

    #16399

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Bacterial Resistance

    Ampicillin and streptomycin

    Species

    Escherichia coli

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. sAJM.1506

    Bacterial Strain

    #108254

    Purpose

    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

13. HL 770

    Bacterial Strain

    #60369

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

14. HL 716

    Bacterial Strain

    #60368

    Purpose

    Lim Lab Strain

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Bacterial Resistance

    None

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. BL21(DE3) ΔserC

    Bacterial Strain

    #197656

    Purpose

    Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Bacterial Resistance

    None

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

16. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

17. Strain SIJ_488

    Bacterial Strain

    #68246

    Purpose

    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Bacterial Resistance

    None

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. USO hisB- pyrF- rpoZ-

    Bacterial Strain

    #18049

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Bacterial Resistance

    Tetracycline

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

19. C321.∆A T7RNAP ompT- rne- lon-

    Bacterial Strain

    #182778

    Purpose

    Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

    Depositor

    Michael Jewett

    Article

    Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

    Bacterial Resistance

    Spectinomycin

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

20. B-95.ΔAΔfabR

    Bacterial Strain

    #197934

    Purpose

    BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

    Bacterial Resistance

    None

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only