Skip to main content
Addgene
Showing: 1 - 20 of 1870 results

  1. lentiCas9-Blast (Lentiviral Prep)

    Viral Prep
    #52962-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Promoter
    EFS-NS
    Tags
    None
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pspCas9-POLI-ST2-com vector

    Plasmid
    #176234
    Purpose
    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace
    Depositor
    Baisong Lu
    Article
    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    176234-plasmid-map-sequence-id-348845

    Enlarge

  7. Cre-responsive Cas9 expressing transposon library vector

    Plasmid
    #164138
    Purpose
    Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)
    Depositor
    Hyongbum Kim
    Article
    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00
    Type
    Empty backbone
    Use
    CRISPR, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, EF-1a
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    164138-plasmid-map-sequence-id-333975

    Enlarge

  8. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Baisong Lu
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    132556-plasmid-map-sequence-id-261537

    Enlarge

  15. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-432916

    Enlarge

  16. dCas9 gateway compatible

    Plasmid
    #128322
    Purpose
    dCas9 C-terminal gateway cloning vector
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128322-plasmid-map-sequence-id-255033

    Enlarge

  17. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid
    #107049
    Purpose
    All-in-one vectors expressing both SaCas9 and LacZ sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107049-plasmid-map-sequence-id-208585

    Enlarge

  18. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid
    #107050
    Purpose
    All-in-one vectors expressing both SaCas9 and YFP sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107050-plasmid-map-sequence-id-208586

    Enlarge

Showing: 1 - 20 of 1870 results