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Plasmid#66102PurposeEntry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertChronos
UseGatewayTagsExpressionMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
FCK-Chronos-GFP
Plasmid#80902PurposeLenti-mediated expression of Chronos-GFP under the CaMKII promoter.DepositorInsertChronos-GFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Chronos-mRuby2-ST
Plasmid#105446PurposeCation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos-mRuby2-ST
UseTagsmRuby2 and soma targeting motifExpressionMutationPromoterpCAGGSAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:Chronos-tdTomato
Plasmid#124232PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomatoDepositorInsertChronos-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet
Plasmid#121542PurposeChronos with red nucleus-targeted fluorescence for excitatory neuronsDepositorInsertblue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
UseAAV and Cre/LoxTagsExpressionMutationPromoterCamKIIAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP
Plasmid#59170PurposeAAV-mediated expression of Chronos-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-tdTomato
Plasmid#62726PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Chronos-GFP
Plasmid#58805PurposeAAV expression of Chronos-GFP under the CaMKII promoterDepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-rc[Chronos-GFP]
Plasmid#59056PurposeExpression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)DepositorInsertChronos-GFP
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Chronos-GFP
Plasmid#99232PurposeAAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-tdTomato
Plasmid#122099PurposeAAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-GFP
Plasmid#122098PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Chronos(M140T)-mRuby2-ST
Plasmid#109129PurposeModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos(M140T)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire
Plasmid#149690PurposeFor packaging and expression of Chronos-mT-Sapphire in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Chronos-tdTomato]
Plasmid#84484PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.DepositorInsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc[Chronos-GFP]
Plasmid#62722PurposeAAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Chronos-mT-Sapphire-NRN
Plasmid#149692PurposeFor packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAVDepositorInsertChronos
UseAAVTagsmT-SapphireExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]
Plasmid#122102PurposeAAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1a(1.1kb short version)Available sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [Chronos-tdTomato]
Plasmid#105834PurposeAAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChronos-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FLEX-rc[Chronos-GFP]
Plasmid#62725PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1αAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only