-
Plasmid#213723PurposeFor overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.DepositorInsertEGFP
UseTagsV5-tag-GSTExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2-ALA
Plasmid#203665PurposeExpresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cellsDepositorInsertZapCV2-Ala zinc dead sensor
UseTagsSV40 NLSExpressionMammalianMutationCys and His in Zap1 is mutated with AlaPromoterCMV promoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP humanAPOBEC1 L173A/G227A
Plasmid#167171PurposeExpresses human APOBEC1 L173A/G227A in mammalian cellsDepositorArticleInserthumanAPOBEC1 (APOBEC1 Human)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)
Plasmid#182369PurposeDendra2-based translation reporterDepositorInsertmtIF3 (Mtif3 Mouse)
UseTagsPal-Dendra2ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_V577D-talin1 (1-2541)
Plasmid#166115Purposeexpresses talin-1-V577D in mammalian cellsDepositorInsertV577D-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_P229L-talin1 (1-2541)
Plasmid#166113Purposeexpresses talin-1-P229L in mammalian cellsDepositorInsertP229L-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTNG5kwh
Plasmid#128279PurposeMammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion proteinDepositorInserthTetR::NLS::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsEGFPExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP773_pLenti-6xHRBKET-sgRNA
Plasmid#211766PurposeMultiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selectionDepositorInsertgRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
UseLentiviralTagsExpressionMutationPromoterpCMV and U6Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF3
Plasmid#69046Purposeexpression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF3 (IKZF3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterIRESAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRF.1 udsVenus (P_JUND)
Plasmid#58692PurposeA rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)DepositorInsertendogenous promoter of JUND (JUND Human)
UseLentiviralTagsudsVenusExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-scFVtet2_bGHpA
Plasmid#177353PurposeAAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…Promoterhuman Synapsine promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg3
Plasmid#127160PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotypeDepositorInsertIghg3 (Ighg3 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla
Plasmid#199551PurposePiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoterDepositorUsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterTRE+CMVmin promoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TERT - WT
Plasmid#213827PurposeExpresses human telomerase reverse transcriptase, the catalytic subunit of telomeraseDepositorInserthuman TERT (TERT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ISDra2-TnpB-mCherry-U6-ωRNA*
Plasmid#212967PurposeExpression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoterDepositorInsertsISDra2-TnpB-T2A-mCherry
ωRNA*
UseCRISPRTagsNLS and NLS-FLAGExpressionMammalianMutationPromoterCAG, T7 and U6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
TERT - R865C
Plasmid#213926PurposeExpresses Mutant TERT R865CDepositorInsertMutant TERT R865C (TERT Human)
UseLentiviralTags6x His TagExpressionMammalianMutationArginine 865 changed to CysteinePromoterTet-responsive promoter PTight, consisting of sev…Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS
Plasmid#209787PurposeExpresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)DepositorInsertTNT4, TadA8e, SpRY N, inteinN, miR122 TS
UseAAVTagsExpressionMutationPromoterTNT4Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1
Plasmid#187288PurposeExpress pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63LDepositorUseTagsmCherryExpressionMammalianMutationAH A740D, E758K, RhoA Q63LPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only