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Plasmid#51240PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 E76A
Plasmid#8331DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationE76A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
retro-gfp-puro vector
Plasmid#58249PurposeRetroviral expression vector encoding bicistronic EGFP and Puromycin cassetteDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe Puro osTIR1-9Myc
Plasmid#80074PurposeRetroviral vector for stable expression of osTIR1-9Myc in mammalian cell linesDepositorInsertOsTIR1 (TIR1 O. sativa)
UseRetroviralTags9 x MycExpressionMutationPromoterGagAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-RUNX3-T2A-PuroR
Plasmid#162349PurposeLentiviral expression of RUNX3 under the control of the TetON promoterDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsT2A-PuroRExpressionMammalianMutationPromoterTet-ONAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-cRAF-W22
Plasmid#15270DepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA CAG
Plasmid#50927PurposeU6 driven sgRNA negative controlDepositorInsertnegative control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…PromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mClover3-Puromycin
Plasmid#167205PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsmClover3ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 1
Plasmid#10671DepositorInsertp53 shRNA (TP53 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro)-Omi-mCherry
Plasmid#48685PurposeExpresses fusion protein in mitochondria inter-membrane space, and is released upon MOMP.DepositorInsertOmi (HTRA2 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterLTRAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-puro-N-Flag-h-SNRNP40
Plasmid#134254PurposeLentivector encoding Flag-tagged human SNRNP40DepositorInsertSNRP40 (SNRNP40 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1aAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PtenG129E-Puro
Plasmid#135678PurposeConstitutive expression (cMV promoter) of G129E mutant Pten cDNA, Puromycin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationG129EPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PtenK13E-Puro
Plasmid#135680PurposeConstitutive expression (cMV promoter) of K13E mutant Pten cDNA, Puromycin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationK13EPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PtenY138L-Puro
Plasmid#135679PurposeConstitutive expression (cMV promoter) of Y138L mutant Pten cDNA, Puromycin selectionDepositorInsertPten (Pten Mouse)
UseLentiviralTagsExpressionMutationY138LPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC118-FLIP-FlpE-Puro
Plasmid#84539Purposea mock vector for generating FLIP-FlpE targeting vectorsDepositorInsertFLIP-FlpE - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro Vps34-GFP
Plasmid#38268DepositorInsertPhosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FlaviA-GFP-puro
Plasmid#140088PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only