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Plasmid#174067PurposeExpresses CIB1 in S. cerevisiae. Includes inducible lac operon.DepositorInsertpGal1-LacO-3NLS-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#174070PurposeExpresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal-Olac-3xNLS-VP16-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL29
Plasmid#174063PurposeExpresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.DepositorInsert2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
UseTagsFLAGExpressionYeastMutationPromoterpCyc1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-REG1-3'UTR
Plasmid#136052PurposeREG1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (ATTGTATCTCTGTAGTTTAAG)DepositorInsertREG1 (ZC3H12A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-lambdaN-UPF1-DEAA
Plasmid#136007PurposeDEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)DepositorInsertUPF1
UseTagsLambdaNExpressionMammalianMutationDE647AA and S743A (Please see depositor comments …PromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF3A-3'UTR
Plasmid#136042PurposeUPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)DepositorInsertUPF3A (UPF3A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF3A-CDS
Plasmid#136043PurposeUPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)DepositorInsertUPF3A (UPF3A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF3B-3'UTR
Plasmid#136044PurposeUPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)DepositorInsertUPF3B (UPF3B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-D1A
Plasmid#127753PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Divergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-C1A
Plasmid#127752PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Convergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMY07AB-PRO
Plasmid#127710PurposeLevel 0 destination vector for a promoter with A - B scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL1259R
Plasmid#118995PurposemCherry (without start and stop codons) in pGFL1010DepositorInsertmCherry (without start and stop codons)
UseFungal expressionTagsExpressionMutationPromoterpromoter lessAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL1260
Plasmid#116900PurposeeGFP:TubA (tubulin marker )DepositorInsertsTubA promoter
TubA (ORF+3'UTR)
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL1260R
Plasmid#116901PurposemCherry:TubA (tubulin marker)DepositorInsertsTubA promoter
TubA (ORF+3'UTR)
UseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
LPhP-pHG165
Plasmid#90047PurposeConstitutive expression of a chimeric LacI:PurR transcription repressorDepositorInsertLPhP
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiN HPV8 E6 K142A
Plasmid#87680PurposeLentiviral expression of HPV8 E6 K142ADepositorInsertHPV 8E6
UseLentiviralTagsFlag and HAExpressionMutationK142APromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiN MmuPV1 E6 R130A
Plasmid#87679PurposeLentiviral expression of MmuPV1 E6 R130ADepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationR130APromoterCMVAvailable sinceMarch 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
NCMV MmuPV1 E6 R52A
Plasmid#87678PurposeFlag/HA tagged MmuPV1 E6 R52ADepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationR52APromoterCMVAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiN MmuPV1 E6
Plasmid#87661PurposeLentiviral expression of MmuPV1 E6DepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only