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sgRNA EMX1
Plasmid#128121PurposeCo-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA EMX1 + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS306:Met3p-mCherry-Tub1
Plasmid#37554DepositorInsertMet3p-mCherry-Tub1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM911
Plasmid#72973PurposeLevel 0 - SJM911 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM911 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
px459-Rheb sgRNA
Plasmid#133768PurposeExpresses Cas9 and human Rheb sgRNADepositorInsertRheb sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS2-FLAG-Sestrin2-E451A
Plasmid#73679PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationE451A changed Glutamate 451 to AlaninePromoterSesn2Available sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAH0016
Plasmid#79824PurposeDual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicolDepositorInsertscat
aadA
UseTagsExpressionMutationPromoterAATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…Available sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2_2a
Plasmid#73010PurposeSecondary module (BpiI) site in pTU2a backboneDepositorInsertSecondary module + Golden Gate pTU2a BsmBI destination site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-lacZ
Plasmid#72936PurposeLevel 1 Destination vector - Position BDepositorInsertLevel 1 - B site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS303:VC
Plasmid#37556DepositorInsertVenus C-term (aa 155-238)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTU3-A-RFP
Plasmid#72945PurposeLevel 3 Destination vector - Assembly of 2 Level 2 modules - ABDepositorInsertLevel 3 - A site
UseSynthetic BiologyTagsExpressionMutationBeta-lactamase - G717C (nucleotide) - silent muta…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B INCENP Δcen mCherry
Plasmid#45227DepositorInsertvsv Cenp-B INCENP Δcen mCherry
UseTagsExpressionMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-miR-315
Plasmid#22765DepositorInsertmicro-RNA 315 (mir-315 primary transcript, Fly)
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-SMCR8
Plasmid#83440PurposeExpresses Cas9 and a gRNA targeting SMCR8DepositorInsertgRNA targeting human SMCR8 (SMCR8 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL6000
Plasmid#216797PurposePlatform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinaseDepositorTypeEmpty backboneUseCre/Lox; Leishmania dicre inducible expressionTags6xHAExpressionMutationPromoterrRNA promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
G1088E_pLenti-CMV-mNeonGreen-2A-HygroR
Plasmid#216279PurposeGreen and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experimentsDepositorInsertmNeonGreen-2A-Hpt
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR
Plasmid#200636PurposeStrong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cellsDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-uTEV1-V5; UAS-mCherry
Plasmid#173122PurposeExpresses uTEV1 and reporter receiver construct in DIPGDepositorInsertArrestin-uTEV1-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-eLOV-TEVcs-Gal4
Plasmid#173119PurposeExpresses CCR6 eLOV receiver construct in DIPGDepositorInsertCCR6-eLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only
