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Plasmid#117545PurposeLevel 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0008
Plasmid#117544PurposeLevel 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0002
Plasmid#117543PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A R181A R182A R186A (N4A)
Plasmid#111940PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutationDepositorInsertHsh155 T178A R181A R182A R186A (N4A)
UseTagsExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A R775A R776A Q778A (C4A)
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
UseTagsExpressionBacterial and YeastMutationQ773A R775A R776A Q778APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT1
Plasmid#67116PurposeStandard for cell-free expression benchmarking.DepositorInsertSTAT1 (STAT1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1L
Plasmid#67096PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2A1L (GTF2A1L Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TP53
Plasmid#67078PurposeStandard for cell-free expression benchmarking.DepositorInsertTP53 (TP53 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IRF9
Plasmid#67079PurposeStandard for cell-free expression benchmarking.DepositorInsertIRF9 (IRF9 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 E-Cad
Plasmid#67046PurposeStandard for cell-free expression benchmarking.DepositorInsertE-Cad (CDH1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCEB2
Plasmid#67049PurposeStandard for cell-free expression benchmarking.DepositorInsertTCEB2 (ELOB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationM6R and Q115KPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 BTF3L4
Plasmid#67051PurposeStandard for cell-free expression benchmarking.DepositorInsertBTF3L4 (BTF3L4 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p207-Switch-ON (FRT)
Plasmid#217886PurposeRetroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoRDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hHSF1 WT
Plasmid#71724Purposeplasmid expressing myc-his tag hHSF1 WTDepositorInsertHSF1 (HSF1 Human)
UseTagsMYC 6xHISExpressionMammalianMutationPromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BRD4
Plasmid#183939PurposeTranscriptional co-activator BRD4 labeled by mCherryDepositorInsertmCherry-BRD4 (Brd4 Mouse, Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTHmut
Plasmid#178950PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cellsDepositorInsertAPOBEC1-YTHmut-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OASL-V5 puro
Plasmid#158643PurposeLentiviral expression vector for constitutive expression of V5-tagged OASL.DepositorInsertOASL (OASL Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OAS1-V5 puro
Plasmid#158641PurposeLentiviral expression vector for constitutive expression of V5-tagged OAS1.DepositorInsertOAS1 (OAS1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralTagsExpressionMutationPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorInsertNuclear Factor I X2 (Nfix Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only