Addgene

We narrowed to 5,711 results for: pCas

Showing: 1901 - 1920 of 5711 results

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pX330-Flag-eSpCas9-D10A

Plasmid

#80449

Purpose

enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

ehSpCas9_D10A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEN396-pCAGGS-TIR1-V5-2A-HygroR

Plasmid

#195102

Purpose

Vector to introduce osTir1 in cells. Hygromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

TIR1

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Human, Synthetic)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

Plasmid

#148855

Purpose

Mammalian Expression of HsAGO3-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO3-sgRNA (AGO3 S.pyogenes)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP-dCas9-mRFP

Plasmid

#102933

Purpose

Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein) and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only
pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-eSpCas9-H840A

Plasmid

#80454

Purpose

enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

ehSpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PA)

Plasmid

#128174

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PA N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-MontiRed-LOV2

Plasmid

#178872

Purpose

Mammalian expression of PB1-MontiRed-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-MontiRed-LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-3m160-HilA

Plasmid

#153325

Purpose

Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-3m160 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and EF1

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM394

Plasmid

#84204

Purpose

Disturb the expression of miR394 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR394

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-iCreERT2 (PV)

Plasmid

#128171

Purpose

Monomeric TagBFP2 and iCre-ERT2 recombinase (PV N-terminus)

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM159

Plasmid

#84195

Purpose

Disturb the expression of miR159 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR159

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only