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Plasmid#173005Purposefirefly luciferase reporter vector with 8x clustered TEAD binding sitesDepositorInsert8xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-27877
Plasmid#202110PurposeLevel 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsert27877 promoter
UseSynthetic BiologyTagsExpressionMutationPromoter sequence mutated to remove sapI sites fo…PromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABR mCherry-Bcd-LEXY
Plasmid#182596PurposeDrosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsLEXY and mCherryExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR Bcd-LEXY
Plasmid#182594PurposeDrosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBicoid (bcd Fly)
UseTagsLEXYExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97E
Plasmid#125825PurposeExpresses GST-RPN13 (19-132) K97E in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to glutamatePromoterT7Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD277
Plasmid#125098PurposeExpression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_2193(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD287
Plasmid#125103PurposeExpression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4637(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD281
Plasmid#125100PurposeExpression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4428(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD285
Plasmid#125102PurposeExpression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4623(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR276.4
Plasmid#125092PurposeExpression of ompR(1-122aa)-psdR(119-237aa) chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsompR(REC)-psdR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR240
Plasmid#125090PurposeExpression of ompR(1-137aa)-psdR(134-237aa) chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsompR(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97A
Plasmid#125823PurposeExpresses GST-RPN13 (19-132) K97A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to alaninePromoterAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD283.2
Plasmid#125101PurposeExpression of SO_4487(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4487(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD275
Plasmid#125097PurposeExpression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_0622(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
gRNA[bTub+DsxF].1046A
Plasmid#112694Purposeexpress two gRNA targeting bTub & DsxF under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut3
Plasmid#53742Purposepromoter region of PIM2 lacking the 2 forkhead binding sitesDepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationdeletion of 2 forkhead binding sites (bp# 395-497…PromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49222PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only