We narrowed to 3,388 results for: golden gate

1. BCJJ339

   Plasmid

   #117501

   Purpose

   RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5a

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ147

   Plasmid

   #69299

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGF002

   Plasmid

   #48843

   Purpose

   Provides a BASTA resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   p35S::BastaR::t35S
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   internal BsaI recognition site in 35S promoter re…

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. BCJJ358

   Plasmid

   #117505

   Purpose

   RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5a

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3749

   Plasmid

   #154108

   Purpose

   loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::his-58::FRT3
   

   Use

   Cre/Lox; Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. L0-I18 Ub-Arg GG1-GG2

   Plasmid

   #195899

   Purpose

   Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   Ub-Arg tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. BCJJ344

   Plasmid

   #117503

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD355 VPR-FRB (NLS)

   Plasmid

   #138847

   Purpose

   Constitutive expression of VPR-FRB under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-FRB
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. L0-I39 PhiC31-Intein GG1-GG4

   Plasmid

   #195905

   Purpose

   Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   PhiC31-intein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. L0-I40 Intein-PhiC31 GG1-GG4

    Plasmid

    #195906

    Purpose

    Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    Intein-PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGGF008

    Plasmid

    #48848

    Purpose

    Provides a BASTA resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pNOS::BastaR::tNOS
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    chi sequence in BastaR removed by silent substitu…

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLY134

    Plasmid

    #130962

    Purpose

    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Insert

    sgRNA-LEB5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson promoter J23100

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPOR1CB0058

    Plasmid

    #117539

    Purpose

    Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (SaCas9)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZA55

    Plasmid

    #174768

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-L17E without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    L17E

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZA56

    Plasmid

    #174769

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-L17E/D481V without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    L17E/D481V

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZA54

    Plasmid

    #174767

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-D481V without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D481V

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZA53

    Plasmid

    #174766

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge