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Plasmid#86611PurposeExpresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmidDepositorInsertATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1L
Plasmid#33242DepositorInsertATXN1L (ATXN1L Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-FLAG-TAOK2
Plasmid#197862PurposeExpression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cellsDepositorInsertTAOK2 (TAOK2 Human)
UseTagsGFPExpressionMammalianMutationWild TypePromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK Smad3N Flag
Plasmid#14827DepositorInsertSmad3 N (SMAD3 Human)
UseTagsFlagExpressionMammalianMutationamino acids 1-146PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-NADK-3xFLAG
Plasmid#163452Purposelentiviral overexpression vector for human NADKDepositorInsertNADK (NADK Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceMarch 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-YOD1
Plasmid#49784PurposeExpress YOD1 with a N-terminal Flag-tag in mammalian cellsDepositorInsertYOD1 (YOD1 Human)
UseTagsFlag-tagExpressionMutationWTPromoterAvailable sinceDec. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag BimL (AA)
Plasmid#24240DepositorInsertBim L(AA) (BCL2L11 Human)
UseTagsFlagExpressionMammalianMutationchanged Threonine 56 to Alanine changed Serine …PromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NmCas9-FLAG
Plasmid#47872PurposeFLAG tagged NmCas9 with no NLS.DepositorInsertNmCas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Ubac2
Plasmid#123597DepositorInsertubiquitin associated domain containing 2 (Ubac2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2 (3A)
Plasmid#101763PurposeExpresses SMAD2 (3A) in mammalian cellsDepositorInsertSMAD2 (SMAD2 Human)
UseTagsFlagExpressionMammalianMutationThree Ser to Ala mutations in the phosphorylation…PromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2-YAP1
Plasmid#19044DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTags2xFlagExpressionMammalianMutationTryptophan 199 changed to Alanine, and Proline 2…PromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Fzd6
Plasmid#123588DepositorInsertfrizzled class receptor 6 (Fzd6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-FLAG-Gene33
Plasmid#21633DepositorInsertGene33 (Errfi1 Rat)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk1a1
Plasmid#15743DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Ctdnep1_D67E-2xFlag
Plasmid#196519PurposeExpression of Ctdnep1_D67E-2xFlagDepositorInsertCtdnep1_D67E-2xFlag (CTDNEP1 Human)
UseTags2xFlagExpressionMammalianMutationD67E mutation (phosphastase dead)PromoterCMV promoter; T7 promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK32-Flag
Plasmid#107432PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK32 (CPK32 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1b2
Plasmid#13752DepositorInsertJnk1b2 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFastBac flag Bmi1
Plasmid#1967DepositorInsertBmi1 (Bmi1 Mouse)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
CDK2-3XFlag-pBabe
Plasmid#73557Purposevector encoding a fusion protein between CDK2 and 3 Flag tagsDepositorInsertCDK2 (CDK2 Human)
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only