HBT-CPK32-Flag
(Plasmid
#107432)
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PurposeFor Arabidopsis mesophyll protoplast transient expression assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerNEB
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCPK32
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1614
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Entrez GeneCPK32 (a.k.a. ATCPK32, CALCIUM-DEPENDENT PROTEIN KINASE 32, CDPK32, CPK32)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HBT-CPK32-Flag was a gift from Jen Sheen (Addgene plasmid # 107432 ; http://n2t.net/addgene:107432 ; RRID:Addgene_107432) -
For your References section:
Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Liu KH, Niu Y, Konishi M, Wu Y, Du H, Sun Chung H, Li L, Boudsocq M, McCormack M, Maekawa S, Ishida T, Zhang C, Shokat K, Yanagisawa S, Sheen J. Nature. 2017 May 18;545(7654):311-316. doi: 10.1038/nature22077. Epub 2017 May 10. 10.1038/nature22077 PubMed 28489820
