-
Plasmid#146012PurposeInsect Expression of DmSmaug_584-999DepositorInsertDmSmaug_584-999 (smg Fly)
UseTagsExpressionInsectMutation5 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSmaug-A642H-V5His6_C
Plasmid#146014PurposeInsect Expression of DmSmaug-A642HDepositorInsertDmSmaug-A642H (smg Fly)
UseTagsExpressionInsectMutation10 silent mutations and a deletion of the AA 961-…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C
Plasmid#146016PurposeInsect Expression of DmSmaug-K606AR609ADepositorInsertDmSmaug-K606AR609A (smg Fly)
UseTagsExpressionInsectMutation10 silent mutations and a deletion of the AA 961-…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-nanos_C
Plasmid#146018PurposeInsect Expression of nanos-3UTRDepositorInsertnanos-3UTR (nos Fly)
UseTagsExpressionInsectMutation3'UTR contains deletions and mutations compa…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmBelle_B
Plasmid#145932PurposeInsect Expression of DmBelleDepositorInsertDmBelle (bel Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1_92-1180-siRNAres-V5His6_N
Plasmid#146990PurposeInsect Expression of DmUPF1_92-1180-siRNAresDepositorInsertDmUPF1_92-1180-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C
Plasmid#146013PurposeInsect Expression of DmSmaug_763-999DepositorInsertDmSmaug_763-999 (smg Fly)
UseTagsExpressionInsectMutation2 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsIRFP and LEXYExpressionInsectMutationPromotermaternal tubulinAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC5Kan-P2A
Plasmid#51814PurposeP2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single messageDepositorInsertP2A peptide (PTV1gp1 porcine teschovirus-1, Synthetic)
UseShuttle vector for insect expression vectorsTagsExpressionMutationcodons altered for Drosophila and to eliminate re…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRTagsExpressionInsectMutationEGFP-D102G see depositor comments belowPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1
Plasmid#22420DepositorInsertArgonaut-1, variant A (AGO1 Fly)
UseDrosophila expressionTagslambdaN-HAExpressionMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorInsertfull length me31B (me31B Fly)
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-CFP-MEK
Plasmid#42077DepositorInsertDrosophila MEK ortholog (Dsor1 Fly)
UseTagsCFPExpressionInsectMutationPromoterHsp70Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGex4t2 with full-length SUUR
Plasmid#112990PurposeExpresses fusion of GST and full-length Drosophila SUURDepositorInsertSuUR (SuUR Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorInsertOrco (Y478A) (Orco Fly)
UseUnspecifiedTagsExpressionMutationchanged 478 Tyrosine for AlaninePromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047
Plasmid#69886PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in DrosophilaDepositorUseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only