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Plasmid#129410PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorInsertsgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Slc1a3 nickase 2
Plasmid#129411PurposeCas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 geneDepositorInsertsgRNA targeting Slc1a3 (Slc1a3 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA
Plasmid#113696PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA
Plasmid#113695PurposeU6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (EGFP) shPnky-2
Plasmid#79142PurposeStable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of CreDepositorInsertshPnky-2 (Gm30731 Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for the shRNA and CMV for EGFPAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lox-Stop-Lox TOPO Hygro
Plasmid#35688DepositorInsertloxP-STOP-loxP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-Rev-FlpO
Plasmid#191209PurposeCre dependent FlpO expression under the control of CAG promoterDepositorInsertFlpO
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPI
Plasmid#12254Purpose2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsEMCV IRES-EGFP cassetteExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-EGFP
Plasmid#59331PurposeExpresses EGFPDepositorHas ServiceAAV1InsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Flex-TREx2-tTA
Plasmid#191210PurposeCre dependent TRE-tTA expression under the control of Syn promoterDepositorInserttTA
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-GFP
Plasmid#28304DepositorInsertGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
BS II KS+ loxP-GFP_ZEO_loxP
Plasmid#128064Purposehas loxp-GFP-Zeo-Loxp cassetteDepositorInsertloxp-GFP-Zeo-Loxp
UseCre/LoxTagsExpressionMutationnoPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
RV-Syn-Dio-yfp_2a_neto1
Plasmid#98176PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-Neto1DepositorInsertNeto1 (Neto1 Mouse)
UseRetroviralTagsExpressionMutationPromoterSynAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
cEF1a-LSL-GFP
Plasmid#135672PurposeIntroduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertGFP
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP
Plasmid#129111PurposeHomologous recombination vector for excision reporter 1 in the CLYBL locus.DepositorInsertLSL, eGFP, NLS-RFP
UseCre/Lox and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Efla_FLEX_APEX2-eGFPf
Plasmid#171939PurposeExpresses a membrane targeted (farnesylation) APEX2-eGFP fusion proteinDepositorInsertAPEX2-eGFPf
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.synP.DIO.EGFP.WPRE.hGH
Plasmid#100043PurposeAn AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorHas ServiceAAV9InsertEGFP
UseAAVTagsExpressionMammalianMutationPromotersynapsin-1Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEZ-Frt-lox-DT
Plasmid#11736DepositorInsertpEZ-Frt-lox-DT
UseCre/Lox; /frtTagsExpressionMutationPromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2
Plasmid#171938PurposeExpresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteinsDepositorInsertApex2, mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only