Addgene

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M26L DJ-1 pET15b

Plasmid

#60679

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26L DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26L

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pDS69

Plasmid

#48367

Purpose

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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psiCheck2 6xmut UTR

Plasmid

#40764

Purpose

Renilla luciferase reporter containing siRNA target sites

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

6 copies (3 site expanded) of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

TK/SV40

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pHJ17

Plasmid

#48356

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHJ22

Plasmid

#48358

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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S9_attL1-EGFP-C-term-attL2

Plasmid

#128473

Purpose

EGFP entry clone

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Use

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PhoDer

Plasmid

#188473

Purpose

Expresses the optogenetic human Gasdermin D (PhoDer) in mammalian cells

Depositor

Gary Mo

Article

Santa Cruz Garcia et al Nat Commun. 2022 Jan 10;13(1):52. doi: 1

Insert

PhoDer (GSDMD Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-VP40

Plasmid

#74421

Purpose

Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

Depositor

Judith White

Article

Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

Insert

VP40

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F4

Plasmid

#37966

Depositor

Jacqueline Lees

Article

Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

Insert

E2F4 (E2F4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-SOS

Plasmid

#208275

Purpose

Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-SOS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Article

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway entry vector

Tags

3xFlag

Expression

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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