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Plasmid#229786PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Convergent
Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent
Plasmid#229800PurposeBxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1aDepositorInsertmGreenLantern; mRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Divergent
Plasmid#229791PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b Ag43 160N 6H mCherry-R5 AmpR
Plasmid#225141PurposeRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.DepositorInsertAg43 160N, mCherry
UseTagsAg43 protein fused to silaffin-R5 and mCherryExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA
Plasmid#222708PurposeExpression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cellsDepositorUseTagsHA, MBP, and mycExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-FCPF
Plasmid#220132PurposeExpresses Cas9-FCPF in E.coliDepositorInsertCas9-FCPF (cas9 S. pyogenes)
UseTagsHis and NLSExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FCPF
Plasmid#220131PurposeExpresses dCas9-FCPF in mammalian cellsDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-FCPF
Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
UseTags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2094_CRY2olig-miRFP
Plasmid#215114PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cellsDepositorInsertCRY2olig-miRFP (CRY2 Mustard Weed)
UseTagsmiRFPExpressionMammalianMutationE490GPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
(R)pGPD1(D)_P1(SBE109)
Plasmid#194993Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)DepositorInsert(R) pGPD (D)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-B'-l2-B'
Plasmid#209119PurposeBacterial expression of CC-GEMS ligand SUMO-B'-l2-B'DepositorInsertSUMO-B'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'
Plasmid#209115PurposeBacterial expression of CC-GEMS ligand SUMO-A'DepositorInsertSUMO-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l3-A'
Plasmid#209118PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l3-A'DepositorInsertSUMO-A'-l3-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-G'
Plasmid#209122PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-G'DepositorInsertSUMO-A'-l2-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l2-B'
Plasmid#209121PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l2-B'DepositorInsertSUMO-A'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l1-A'
Plasmid#209116PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l1-A'DepositorInsertSUMO-A'-l1-A'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-G'-l2-G'
Plasmid#209120PurposeBacterial expression of CC-GEMS ligand SUMO-G'-l2-G'DepositorInsertSUMO-G'-l2-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only