1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. tRNA-gRNA position [D1_n-1] (GB1208)

   Plasmid

   #75409

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [D1_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. tRNA-gRNA position [n] (GB1207)

   Plasmid

   #75408

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [n]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMVTNT-EPHX1

   Plasmid

   #53114

   Purpose

   In vitro translation of microsomal epoxide hydrolase

   Depositor

   Xu Lin

   Article

   Chen et al BMC Cancer. 2014 Apr 23;14:282. doi: 10.

   Insert

   EPHX1 (EPHX1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #165492

   Purpose

   Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   darkVenus

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGB 35s:dCas:EDLL:tNos (GB1190)

   Plasmid

   #75404

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional Activator

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas:EDLL
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. tRNA-gRNA position [2_n-1] (GB1206)

   Plasmid

   #75407

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [2_n-1]
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tRNA-gRNA position [D1_2] (GB1205)

   Plasmid

   #75406

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGB 35s:dCas:VP64:tNos (GB1189)

   Plasmid

   #75403

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas:VP64
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 RL3m6L2

   Plasmid

   #109366

   Purpose

   Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO His6 SUMO SnRK2.3 (19-361)

    Plasmid

    #177846

    Purpose

    Bacterial Expression of SnRK2.3

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SITS-EGFP-Bin1a

    Plasmid

    #176020

    Purpose

    Leishmania cell free expression of zebrafish EGFP-Bin1a. Parton lab clone GRQ

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SITS-mCherry-Bin1a

    Plasmid

    #176024

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1a. Parton lab clone GSS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1a
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDECKO_mCherry_KLF4

    Plasmid

    #140104

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid

    #126651

    Purpose

    B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Lrig1 (Lrig1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    C-terminal fusion of T2A-sfGFP-iCre-ERT2

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

    Plasmid

    #117663

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT2)-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-shGBP1.2.mKO2

    Plasmid

    #85210

    Purpose

    TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSYC-190

    Plasmid

    #178962

    Purpose

    Expresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cells

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Insert

    Glial fibrillary acidic protein (GFAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R79C

    Promoter

    CMV IE94 Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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