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Showing: 1861 - 1880 of 1983 results

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  4. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  5. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid
    #75409
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [D1_n-1]
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75409-plasmid-map-sequence-id-211946

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  6. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48348-plasmid-map-sequence-id-70559

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  9. pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #165492
    Purpose
    Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity
    Depositor
    Thomas Oertner
    Article
    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    darkVenus
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165492-plasmid-map-sequence-id-330311

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  12. tRNA-gRNA position [D1_2] (GB1205)

    Plasmid
    #75406
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75406-plasmid-map-sequence-id-146004

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  14. pcDNA3 RL3m6L2

    Plasmid
    #109366
    Purpose
    Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag
    Depositor
    Robert Lucas
    Article
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Insert
    Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
    Use
    Tags
    1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109366-plasmid-map-sequence-id-210142

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Showing: 1861 - 1880 of 1983 results