Addgene

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_HDLBP

Plasmid

#154448

Purpose

For recombination of HDLBP

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

HDLBP (HDLBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-SARS-CoV-2-3CL-C145A

Plasmid

#160279

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain

Plasmid

#79554

Purpose

Expression of catalytic domain of mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mutation

aa990-1912

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pLEX301-ARF6-TagRFP

Plasmid

#162029

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pLEX307-IBV-3CL

Plasmid

#160288

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA1

Plasmid

#89719

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA1 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+10A)-IDR-mCherry

Plasmid

#145282

Purpose

Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+10A)-IDR (HOXD13 Human)

Use

Tags

Expression

Bacterial

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX301-ARF6-T157N-TagRFP

Plasmid

#162030

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

T151N

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(8G)-mCherry

Plasmid

#206950

Purpose

Mammalian expression of heterobifunctional SasG linkers (8G)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (8G)-mCherry

Use

Tags

mCherry and sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX335A_hCas9(D10A)_IRF8gRNA2

Plasmid

#89720

Purpose

Knockout IRF8 in human cells

Depositor

Martin Zenke

Article

Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

Insert

IRF8 gRNA2 (IRF8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-MED1trunc-IDR-YFP-NLS

Plasmid

#145275

Purpose

Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR truncated (MED1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL

Plasmid

#160286

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mCerulean

Plasmid

#185807

Purpose

Mitochondrial targetted (COX8) mCerulean

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mCerulean

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-mCherry-CRY2-NLS

Plasmid

#145260

Purpose

Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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