1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pLM2_30_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS

   Plasmid

   #154222

   Purpose

   Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbC

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVE2_5_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_5xQUAS

   Plasmid

   #154232

   Purpose

   Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_pcbAB

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLM2_35_p40S_QfDBD_Qf2AD_cp_nirA_DsRed_5xQUAS

   Plasmid

   #154244

   Purpose

   Encodes p40S:QfDBD-QF2AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_nirA

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-Qf2AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLM2_40_pgndA_QfDBD_VP16AD_cp_ura3_DsRed_5xQUAS

   Plasmid

   #154240

   Purpose

   Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_ura3

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   pgndA:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   pGndA (An11g02040 A. niger)

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVE2_7_p40S_QfDBD_VP16AD_cp_phl_DsRed_5xQUAS

   Plasmid

   #154234

   Purpose

   Encodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS+CP_phl

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   p40S:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   p40S (AN0465 A. nidulans)

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLM2_45_pgndA_QfDBD_VP16AD_cp_pcbC_DsRed_0xQUAS

   Plasmid

   #154225

   Purpose

   Encodes pgndA:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter

   Depositor

   Arnold Driessen

   Article

   Mozsik et al Microb Cell Fact. 2019 Nov 18;18(1):203.

   Insert

   pgndA:QfDBD-VP16AD-GFP-NLS
   

   Use

   Synthetic Biology; Fungal expression

   Tags

   GFP-NLS; DsRed-SKL

   Expression

   Bacterial

   Mutation

   Promoter

   pGndA (An11g02040 A. niger)

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB6-Lrig1-T2A-tdTomato-FNF-TK

   Plasmid

   #134320

   Purpose

   B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Lrig1 (Lrig1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tdTomato

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB6-Cdk6-T2A-td-sfGFP-FNF-TK

   Plasmid

   #134321

   Purpose

   B6J Cdk6-T2A-td-sfGFP allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tandem dimer-superfolder…

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ-pre-Ost1-pro-alphaf-Btl2

   Plasmid

   #117665

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-pro-alphaf-Btl2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-K0XCK7

    Plasmid

    #103118

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    K0XCK7(Cas9 coding gene from Barnesiella intestinihominis YIT 11860)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HeFm1SpCas9

    Plasmid

    #92110

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB3omega1R Tnos:NptII:Pnos-SF (GB1181)

    Plasmid

    #75471

    Purpose

    Transcriptional unit for kanamycin resistance gene

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    NptII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Agrobacterium tumefaciens Nopaline synthase promo…

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

    Plasmid

    #216247

    Purpose

    Express OsTIR1.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    OsTIR1.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Mouse, Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSH-CAG-AtAFB2.F74A-IRES-puro

    Plasmid

    #216245

    Purpose

    Express AtAFB2.F74A mutant without tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Raichu-OsRac1 CA

    Plasmid

    #133264

    Purpose

    Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(G19V) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 G19V

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Raichu-OsRac1 DN

    Plasmid

    #133263

    Purpose

    Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

    Depositor

    Yoji Kawano

    Article

    Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

    Insert

    OsRac1(T24N) (LOC4325879 Oryza sativa)
    

    Use

    Tags

    CFP-lipid and Venus-CRIB

    Expression

    Plant

    Mutation

    OsRac1 T24N

    Promoter

    Ubi

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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