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Plasmid#97012PurposeRosa26 targeting vector for intersectional Cre and FLP responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE
Plasmid#196678PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.DepositorInsertPKIαmut-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS(Luc)-GFP
Plasmid#197887PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-Kir2.1
Plasmid#125578PurposeCre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
R26TV LSL-TRL
Plasmid#68476PurposeRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassetteDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseCre/Lox, Luciferase, and Mouse TargetingTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJEP307-pAAV-EFS(No AgeI)-MCS3-pA
Plasmid#113684PurposeEFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.DepositorInsertN/A
UseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-Mac-GFP
Plasmid#58852PurposeAAV-mediated expression of Mac-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertMac-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-IN
Plasmid#70071Purpose2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cellsDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS5-TVA-eGFP-N2cG
Plasmid#175440PurposeCre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoerDepositorInsertTVA-P2A-eGFP-P2A-N2cG
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-Mac-GFP
Plasmid#58803PurposeAAV-mediated expression of Mac-GFP under the CAG promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertMac-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FLAG_hSF3B1
Plasmid#176590PurposeAdenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1DepositorInsertSF3B1 (SF3B1 Human)
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
Plasmid#199580PurposeExpresses GCaMP8f in a Cre-dependent mannerDepositorInsertHis-DIO GCaMP8f
UseAAV, CRISPR, and Cre/LoxTags6xHisExpressionMammalianMutationPromoterhsynAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
Plasmid#199582PurposeExpresses Chronos-eGFP in a Cre-dependent mannerDepositorInsertChronos eGFP
UseAAV, CRISPR, and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1a intron AAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH
Plasmid#131684PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1DepositorInsertsEGFP-KASH
SpCas9 sgRNA vs mouse GRID1
UseAAVTagsKASHExpressionMutationPromoterEF1a and mU6-LSL (Cre dependent)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-col2a1a-KI-donor
Plasmid#184876PurposeDonor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsCollagen2a1a 5' homology arm
Collagen2a1a 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro
Plasmid#192875PurposeCre-dependent dual reporterDepositorInsertNanoluc-P2A-copGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-Rev-3xGFP
Plasmid#122500PurposeCre dependent GFP expression.DepositorInsert3x GFP
UseAAVTagsExpressionMutationeach GFP has unique nt sequencePromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only